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snapf tag  (New England Biolabs)


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    New England Biolabs snapf tag
    Snapf Tag, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 333 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/snapf tag/product/New England Biolabs
    Average 96 stars, based on 333 article reviews
    snapf tag - by Bioz Stars, 2026-05
    96/100 stars

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    Workflow for expression of pCS2+/SNAPf- and Halo-tagged constructs in Xenopus embryos pCS2+/SNAPf- and Halo-vectors that were codon optimized for Xenopus can be acquired from Addgene. The sequence of interest is cloned into the multiple cloning site (MCS), located upstream/downstream of the SNAPf/Halo-tag. Tagged constructs are in vitro transcribed to generate mRNA that is microinjected into 2–4 cell stage Xenopus embryos.

    Journal: STAR Protocols

    Article Title: SNAP- and Halo-tagging and dye introduction protocol for live microscopy in Xenopus embryos

    doi: 10.1016/j.xpro.2022.101622

    Figure Lengend Snippet: Workflow for expression of pCS2+/SNAPf- and Halo-tagged constructs in Xenopus embryos pCS2+/SNAPf- and Halo-vectors that were codon optimized for Xenopus can be acquired from Addgene. The sequence of interest is cloned into the multiple cloning site (MCS), located upstream/downstream of the SNAPf/Halo-tag. Tagged constructs are in vitro transcribed to generate mRNA that is microinjected into 2–4 cell stage Xenopus embryos.

    Article Snippet: Workflow for expression of pCS2+/SNAPf- and Halo-tagged constructs in Xenopus embryos pCS2+/SNAPf- and Halo-vectors that were codon optimized for Xenopus can be acquired from Addgene.

    Techniques: Expressing, Construct, Sequencing, Clone Assay, In Vitro

    Journal: STAR Protocols

    Article Title: SNAP- and Halo-tagging and dye introduction protocol for live microscopy in Xenopus embryos

    doi: 10.1016/j.xpro.2022.101622

    Figure Lengend Snippet:

    Article Snippet: Workflow for expression of pCS2+/SNAPf- and Halo-tagged constructs in Xenopus embryos pCS2+/SNAPf- and Halo-vectors that were codon optimized for Xenopus can be acquired from Addgene.

    Techniques: Recombinant, Software, Microscopy